Providing you the best range of qiagen qiaamp viral rna mini kit, qiagen mircury lna mirna pcr assay, qiagen quantitect primer assay (200), qiagen qiacube ht plasticware, qiagen 8-rod covers and qiagen dntp set, pcr grade, 4 x 100 with effective & timely delivery.
Qiagen QIAamp Viral RNA Mini Kit
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Product Details:
Brand
Qiagen
Product Type
RNA Kits
CAT No
52904, 52906
Application
Molecular Reagent
Pack Size
50, 250 Nos
QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect using the QIAamp Viral RNA Mini Kit or dedicated QIAamp Viral RNA Mini QIAcube Kit. Various options for automation of the QIAamp 96 Viral Kit on instruments including the QIAcube HT, the Fluent 780 from Tecan or the epMotion 5075V from Eppendorf support high to ultra-high throughput processing with minimal hands-on time.
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Qiagen miRCURY LNA miRNA PCR Assay
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Product Details:
Brand
Qiagen
Product Type
miRNA Kit
Pack Size
1 pg total RNA
CAT No
339306
Application
Molecular Reagent
miRCURY LNA miRNA PCR Assays are individual miRNA PCR primer sets that enable extremely sensitive and specific miRNA quantification with the miRCURY LNA miRNA PCR System. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized with LNA technology. Assays are available in tube or plate format with 200 reactions per tube or well.
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Qiagen QuantiTect Primer Assay (200)
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Product Details:
Brand
Qiagen
Product Type
Primer Assay
Pack Size
200 x 50 ul
CAT No
249900
Application
Molecular Reagent
QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.
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Qiagen QIAcube HT Plasticware
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Product Details:
Brand
Qiagen
Product Type
Nanoplate
Pack Size
120 x 8-Well
CAT No
950067
Application
PCR Plasticware
QIAcube HT Accessories and Consumables are for use with QIAcube HT instruments and provide simple and reliable mid- to high-throughput nucleic acid purification in 96-well format from a range of starting sample materials.
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Qiagen 8-Rod Covers
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Product Details:
Brand
Qiagen
Product Type
8-Rod Covers
Pack Size
144 Nos
CAT No
997004
Product class
PCR Accessories
QIAsymphony SP/AS Accessories are for use with the QIAsymphony SP/AS instruments for automated sample preparation and assay setup. When ordering adapters, ensure that you order the correct adapter for the software version that you are using.
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Qiagen dNTP Set, PCR Grade, 4 x 100
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Product Details:
Brand
Qiagen
Product Type
PCR and RT-PCR Kit
Pack Size
4 x 100 micro liter
CAT No
201912
Application
Molecular Reagent
dNTP Set, PCR Grade is a complete set of individual, highly pure dNTPs. Each individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP to the desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP in water (pH 7.5), each at a concentration of 10 mM. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.
The CGT Viral Vector Lysis Kit is provided with reagents for 100 or 1000 reactions in two boxes for AAV vectors. The kit is suitable for the lysis of AAV2, AAV5, AAV6, AAV8 and AAV9. The lysates are optimized for QIAcuity Cell and Gene Therapy dPCR Assays in combination with the QIAcuity Probe PCR Kit. These assays enable singleplex as well as multiplex cell and gene therapy applications, including viral titer and vector copy number measurements. The kit does not include restriction enzymes (for e.g., Hpall) that are stated in the protocol.
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Qiagen dNTP Set, PCR Grade, 4 x 250
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Product Details:
Brand
Qiagen
Product Type
PCR and RT-PCR Kit
Pack Size
4 x 100 micro liter
CAT No
201912
Application
Molecular Reagent
dNTP Set, PCR Grade is a complete set of individual, highly pure dNTPs. Each individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP to the desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP in water (pH 7.5), each at a concentration of 10 mM. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.
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Qiagen dPCR CGT Assay GFP (FAM)
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Product Details:
Brand
Qiagen
Product Type
PCR and RT-PCR Kit
Pack Size
500 x 12 micro liter
CAT No
250236
Application
Molecular Reagent
Adeno-associated virus (AAV) is a widely used viral vector in gene therapy applications. However, the generation and purification of the viral vectors require rigorous quality control to enable safe and reliable dosing during clinical studies or patient care. The ability to accurately quantify vector titers and detect contamination is critical for safe and effective AAV-based gene therapies.
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Qiagen dPCR CNV Probe Reference Assay
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Product Details:
Brand
Qiagen
Product Type
dPCR Kit
Pack Size
500 x 12 micro liter
CAT No
250213
Application
Molecular Reagent
dPCR CNV Probe Assays enable specific, accurate, reproducible and easy-to-interpret copy number change analysis for individual genes or regions of interest. Assays for more than 200 targets have been PCR wet-lab-validated and are flagged as such in the particular assay description.
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Qiagen dPCR Copy Number Assay (200)
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Product Details:
Brand
Qiagen
Product Type
dPCR Kit
CAT No
250205
Pack Size
200 x 12 ul
Application
Molecular Reagent
dPCR Copy Number Assays enable specific, accurate, reproducible and easy-to-interpret copy number change analysis for an individual gene or region of interest. Assays for more than 200 targets have been dPCR wet-lab tested. All other in silico designs have been bench-verified and are ready to use in NGS follow-up studies, specific target screening and other related studies.
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Qiagen dPCR LNA Mutation Assay (200)
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Product Details:
Brand
Qiagen
Product Type
dPCR Kit
Pack Size
200 x 40 ul
CAT No
250200
Application
Molecular Reagent
dPCR LNA Mutation Assays enable detection of individual sequence mutations selected from comprehensive curated databases such as COSMIC. The assay designs have been dPCR wet-lab tested, and the resulting data is available. The sensitivity for detecting the target mutation is 0.1% in a wild-type background in one well of a QIAcuity Nanoplate. Even higher sensitivity can be achieved by splitting the reaction into multiple wells and combining the analysis. The choice of two different fluorescent dye combinations allows detection of mutant and wild-type sequences as well as multiplexing analysis of two target mutations in one well.
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Qiagen EpiTect MethyLight PCR +ROX Vial Kit (200)
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Product Details:
Brand
Qiagen
Product Type
dPCR Kit
Pack Size
200 x 50 ul
CAT No
59496
Application
Molecular Reagent
The EpiTect MethyLight PCR Kit and the EpiTect MethyLight PCR+ROX Vial Kit enable sensitive and reliable quantification for TaqMan® or dual-labeled probe-based methylation analysis. The kits are available in a ready-to-use format, eliminating the need for further optimization. The kits are compatible with a wide range of real-time cyclers. The EpiTect MethyLight PCR Kit is suitable for most cyclers from Applied Biosystems, with the exception of the Applied Biosystems 7500 systems. The EpiTect MethyLight PCR+ROX Vial Kit is suitable for all other cyclers, including the Applied Biosystems 7500 and instruments from Bio-Rad, Cepheid, Corbett, Eppendorf, Roche, and Stratagene.
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Qiagen Microbial DNA qPCR Assay Kit
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Product Details:
Brand
Qiagen
Product Type
RT PCR Kit
Pack Size
20 ul
CAT No
330033
Application
Molecular Reagent
Microbial DNA qPCR Assay Kits are a complete solution for real-time PCR-based microbial detection. Microbial DNA qPCR Assay Kits contain a Microbial DNA qPCR Assay, which is a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enables quantitative real-time PCR for 20 reactions. The kit also contains everything required for a successful qPCR run, including Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water, and Microbial qPCR Mastermix. What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).
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Qiagen RT2 Profiler PCR Array
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Product Details:
Brand
Qiagen
Product Type
RNA Kits
Pack Size
96 Wells
CAT No
330231
Application
Molecular Reagent
RT² Profiler PCR Arrays enable quick, reliable gene expression analysis of 170+ pathways, allowing researchers to spend less time at the bench and more time interpreting results. The arrays are pathway-focused panels of laboratory-verified qPCR assays, with integrated, patented controls to ensure a successful experiment every time. PhD-trained application specialists are available to provide technical support for the arrays, and each array can also be modified to suit unique experimental needs. For biomarker discoveries, we offer Pathway Plus PCR arrays and all arrays listed below for the Fluidigm BioMark Real-Time PCR System.
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Qiagen miRCURY LNA SYBR Green PCR Kit
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
200T
CAT No
339345
Application
Molecular Reagent
The miRCURY LNA SYBR Green PCR Kit contains a high-performance PCR master mix specifically designed for use with the miRCURY LNA miRNA PCR System. The miRCURY SYBR Green PCR Kit also features a built-in control for visual identification of correct template addition, as well as Q-Bond, an additive in the PCR buffer that enables short cycling steps without loss of PCR sensitivity and efficiency. The kit has been optimized for use with any real-time cycler. ROX Reference Dye is provided in a separate tube and can be added if your cycler requires ROX as a passive reference dye.
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Qiagen QIAcuity EG PCR Kit (1 ml)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
3 x 1 ml
CAT No
250111
Application
Molecular Reagent
The QIAcuity EG PCR Kit contains a 3x concentrated, ready-to-use Master Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit improves the specificity and efficiency of dye-based digital PCR to provide accurate quantitation analysis. An intercalating dye EvaGreen binds to double-stranded DNA and enhances the quantitative accuracy of gDNA or cDNA measurements on the QIAcuity dPCR instruments.
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Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml)
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Product Details:
Brand
Qiagen
Product Type
RT PCR Kit
Pack Size
1 ml
CAT No
250131
Application
Molecular Reagent
The QIAcuity OneStep Advanced Probe PCR Kit enables sensitive quantification of RNA or RNA+DNA targets on the QIAcuity digital PCR instrument in one combined RT-PCR reaction. It contains a 4x concentrated Master Mix and a 100x concentrated RT Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit enhances the specificity and efficiency of probe-based digital PCR to provide accurate, singleplex or up to 5-plex analysis.
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Qiagen QIAcuity Probe PCR Kit (5 mL)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
5 ml
CAT No
250102
Application
Molecular Reagent
The QIAcuity Probe PCR Kit contains a 4x concentrated, ready to use Master Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit enhances the specificity and efficiency of probe-based digital PCR to provide accurate, singleplex, or up to 5-plex analysis. The QIAcuity Probe Master Mix is optimized to increase specificity for accurate quantification of gDNA or cDNA on the QIAcuity dPCR instruments.
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Qiagen QuantiNova LNA PCR Assay (750)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
750T
CAT No
249992
Application
Molecular Reagent
QuantiNova LNA PCR Assays are setting new standards in SYBR® Green-based qPCR gene expression analysis. Choose from the broadest selection of predesigned assays for accurate and sensitive detection of any human, mouse or rat mRNA or lncRNA – from general transcript detection to differentiation of specific transcript isoforms. LNA enhancement allows us to design shorter primers, which are more flexible to position on the target. As a result, we can optimize our designs for specific transcript detection or target differentiation.
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Qiagen QuantiNova Probe PCR Kit (2500)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
2500T
CAT No
208256
Application
Molecular Reagent
Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.
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Qiagen QuantiTect Virus Kit (200)
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Product Details:
Brand
Qiagen
Product Type
viral RNA and/or DNA
Pack Size
200T
CAT No
211013
Application
Molecular Reagent
The QuantiTect Virus Kit is specially designed for highly sensitive detection of up to 4 viral nucleic acid targets by multiplex, real-time one-step RT-PCR using sequence-specific probes. Multiple viral RNA and/or DNA targets plus internal controls can be detected in a single tube without loss of sensitivity. The concentrated 5x master mix enables the addition of higher sample input and contains HotStarTaq Plus DNA Polymerase. The QuantiTect Virus RT Mix contains a unique formulation of Sensiscript Reverse Transcriptase that is optimized for highly sensitive detection of viral RNA. Two kit formats are available: the QuantiTect Virus Kit for cyclers that require ROX dye for fluorescence normalization and the QuantiTect Virus +ROX Vial Kit for other cyclers. For convenience, the master mix can be stored at 2–8°C.
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Qiagen QuantiFast Pathogen RT-PCR +IC Kit (400)
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Product Details:
Brand
Qiagen
Product Type
RT PCR Kit
Pack Size
400 x 25 ul
CAT No
211454
Application
Molecular Reagent
The QuantiFast Pathogen +IC Kits are designed for sensitive and rapid real-time PCR or one-step RT-PCR detection of pathogen nucleic acids using sequence-specific probes. To enable high process safety through correct interpretation of negative detection results, each kit contains reagents for multiplex real-time detection of up to 4 user-defined pathogen targets (e.g., virus, bacteria, fungi etc.) plus the Internal Control (IC). Two kit formats are available: The QuantiFast Pathogen RT-PCR +IC Kit for detection of viral RNA, which contains an internal RNA control template, plus the Internal Control primer/probe set, or the QuantiFast Pathogen PCR +IC Kit for detection of viral, bacterial, or fungal DNA, which contains an internal DNA control template, plus the Internal Control primer/probe set. With both kits, ROX is supplied in 2 tubes of different concentrations, enabling use on virtually any real-time instrument. For convenience, the master mix can be stored at 2–8°C.
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Qiagen QuantiNova Probe PCR Kit (100)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
100T
CAT No
208252
Application
Molecular Reagent
Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.
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Qiagen QuantiTect Rev. Transcription Kit (50)
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Product Details:
Brand
Qiagen
Product Type
Transcription Kit
Pack Size
50T
CAT No
205311, 205313
Application
Molecular Reagent
The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect Reverse Transcription Kit, including Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, and a unique RT Primer Mix. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts.
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Qiagen RT2 SYBR Green Fluor qPCR Mastermix (24)
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Product Details:
Brand
Qiagen
Product Type
qPCR Kit
Pack Size
24 x 1.35 ml
CAT No
330513
Application
Molecular Reagent
RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.
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Qiagen RT2 SYBR Green ROX qPCR Mastermix (24)
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Product Details:
Brand
Qiagen
Product Type
qPCR Kit
Pack Size
24 x 1.35 ml
CAT No
330523
Application
Molecular Reagent
RT2 SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT2 qPCR Primer Assays, RT2 Profiler PCR Arrays, RT2 lncRNA qPCR Assays, and RT2 lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT2 SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.
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Qiagen Type-it Fast SNP Probe PCR Kit (800)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
800 x 25 ul
CAT No
206045
Application
Molecular Reagent
The Type-it Fast SNP Probe PCR Kit is based on highly specific HotStarTaq Plus DNA Polymerase and a newly developed buffer system, both of which enable reliable and clear allelic discrimination. The combination of all components provided in the master mix result in improved accuracy and highly specific probe binding. The Type-it Fast SNP Probe PCR Kit is validated using TaqMan® SNP Genotyping Assays and enables reproducible SNP genotyping — even with difficult SNP loci (e.g., GC rich) or low amounts of starting template.
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Qiagen Type-it HRM PCR Kit (400)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
400 x 25 ul
CAT No
206544
Application
Molecular Reagent
The Type-it HRM PCR Kit provides a reliable solution for fast and accurate genotyping via HRM analysis. The kit is optimized to even enable successful analysis of genomic loci that are difficult to amplify. The kit is highly suitable for mutation scanning and can be used to screen samples for unknown mutations. Development of new HRM genotyping assays is easy and straightforward. Specific amplification products, reduced nonspecific amplification, and reliable results are consistently ensured — time-consuming optimization of PCR parameters is no longer required. This leads to standardization and flexibility, in addition to substantial time and cost savings. The kit is compatible with all real-time instruments suitable for HRM analysis, such as the Rotor-Gene Q, the Rotor-Gene 6000, the LightCycler 480, and the Applied Biosystems 7500 Fast System.
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Qiagen Hot StarTaq Master Mix Kit (2500 U)
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Product Details:
Brand
Qiagen
Product Type
Master Mix
Pack Size
2500 ul
CAT No
203446
Application
Molecular Reagent
HotStarTaq Master Mix contains HotStarTaq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, and dNTPs. Providing all components in a master mix reduces pipetting steps and the risk of contamination, while increasing throughput and reproducibility.
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Qiagen MinElute 96 UF PCR Purification Kit (24)
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
24T
CAT No
28053
Application
Molecular Reagent
The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products ≥100 bp in size. The plates are designed to allow elution in very small volumes (as little as 20 μl) delivering high yields of highly concentrated DNA. The procedure can be automated on the BioRobot Universal System. DNA fragments purified with the MinElute system are ready for direct use in many applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute 96 UF PCR Purification Kit requires the use of the QIAvac Multiwell vacuum manifold (cat. no. 9014579).
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Qiagen Ni-NTA Superflow
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
500 mL
CAT No
30450
Application
Molecular Reagent
Ni-NTA Superflow is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.
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Qiagen Puregene Blood Kit (1000 ml)
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
1000 mL
CAT No
158026
Application
Molecular Reagent
Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.
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Qiagen Puregene Tissue Kit (33 g)
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
33g
CAT No
158066
Application
Molecular Reagent
Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.
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QIAamp 96 Virus QIAcube HT Kit (5)
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Product Details:
Brand
Qiagen
Product Type
PCR Kits
Pack Size
5 x 96 Wells
CAT No
57731
Application.
Molecular Reagent
The QIAamp 96 Virus QIAcube HT Kit enables simple, automated purification of viral RNA and DNA on the QIAcube HT system. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis.
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Qiagen QIAquick 96 PCR Purification Kit (24)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
24 x 96 Wells
CAT No
28183
Application
Molecular Reagent
QIAquick 96 PCR Purification Kits provide 96-well plates, buffers, and collection tubes for high-throughput silica-membrane-based purification of PCR products >100 bp in size. DNA up to 10 kb is purified using a simple and fast bind–wash–elute procedure and an elution volume of 60–80 µl (resulting in an eluate volume of 40–60 µl). The cleanup procedure can be fully automated on the BioRobot Universal workstation using the QIAquick 96 PCR BioRobot Kit.
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Qiagen QuantiFast Multiplex PCR Kit (400)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
400T
CAT No
204654
Application
Molecular Reagent
QuantiFast Multiplex PCR Kits enable fast and reliable quantification of up to 4 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote fast multiplex real-time PCR, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. Two kit formats are available: the QuantiFast Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiFast Multiplex PCR +R Kit for all other cyclers. For convenience, the master mix can be stored at 2–8°C.
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Qiagen REPLI-g Cell WGA & WTA Kit (48)
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Product Details:
Brand
Qiagen
Product Type
WTA Kit
Pack Size
48 Wells
CAT No
150054
Application
Molecular Reagent
The REPLI-g Cell WGA & WTA Kit uniquely enables uniform whole genome amplification (WGA) and whole transcriptome amplification (WTA) in parallel reactions, allowing direct correlation of the genome with the transcriptome from very small samples (25–1000 cells). Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method. The innovative lysis buffer effectively stabilizes cellular nucleic acids, ensuring accurate representation of both genomic DNA and RNA levels. Unbiased amplification of genomic DNA and total RNA or mRNA (poly A+) is achieved using innovative Multiple Displacement Amplification (MDA) technology and the novel REPLI-g SensiPhi DNA Polymerase. In contrast to PCR-based amplification technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.
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Qiagen REPLI g Midi Kit (100)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
100T
CAT No
150045
Application
Molecular Reagent
The REPLI-g Mini and Midi Kits offer a single tube workflow with higher yields of amplified DNA, while the REPLI-g UltraFast Kit enables highly uniform and accurate whole genome amplification in just 60–90 minutes. For high-throughput processing in 96-well format, the streamlined, room-temperature procedure of the REPLI-g Screening Kit enables straightforward liquid handling that requires just 20 minutes. The REPLI-g Screening kit is intended for rapid screening using a range of genotyping, PCR, sequencing or microarray assays.
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Qiagen REPLI-g Screening Kit (200)
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Product Details:
Brand
Qiagen
Product Type
PCR Kit
Pack Size
200T
CAT No
150126
Application
Molecular Reagent
Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). REPLI?g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. QIAGEN REPLI-g Kits are available in several different sizes and configurations which allows researchers to build the best workflow for their application and lab space. Typical REPLI-g yields range from approx. 7 µg to 40 µg of amplified DNA, depending on which kit and workflow is used.
